Rice Galaxy: An open resource for plant science

Background: Rice molecular genetics, breeding, genetic diversity, and allied research (such as rice-pathogen interaction) have adopted sequencing technologies and high-density genotyping platforms for genome variation analysis and gene discovery. Germplasm collections representing rice diversity, improved varieties, and elite breeding materials are accessible through rice gene banks for use in research and breeding, with many having genome sequences and high-density genotype data available. Combining phenotypic and genotypic information on these accessions enables genome-wide association analysis, which is driving quantitative trait loci discovery and molecular marker development. Comparative sequence analyses across quantitative trait loci regions facilitate the discovery of novel alleles. Analyses involving DNA sequences and large genotyping matrices for thousands of samples, however, pose a challenge to non−computer savvy rice researchers. Findings: The Rice Galaxy resource has shared datasets that include high-density genotypes from the 3,000 Rice Genomes project and sequences with corresponding annotations from 9 published rice genomes. The Rice Galaxy web server and deployment installer includes tools for designing single-nucleotide polymorphism assays, analyzing genome-wide association studies, population diversity, rice−bacterial pathogen diagnostics, and a suite of published genomic prediction methods. A prototype Rice Galaxy compliant to Open Access, Open Data, and Findable, Accessible, Interoperable, and Reproducible principles is also presented. Conclusions: Rice Galaxy is a freely available resource that empowers the plant research community to perform state-of-the-art analyses and utilize publicly available big datasets for both fundamental and applied science

Sa piling (at one\u27s side) a short feature on a loving mother who believes that her daughter is an impostor

This short feature intends to show that anyone is susceptible to harm, no matter how stable ones life may seem to be. It also shows how others are greatly affected by someone who is suffering from a mental-illness, in the way that they experience instability despite not being the one who is mentally-ill. Sophia, a 32-year old mother, is desperately looking for her 8 year-old daughter, Sab. On the other hand, Sophia\u27s husband, Paolo, is uncaring about the fact that their daughter has been missing, which makes Sophia feel like Paolo has something to do with Sabs disappearance. Sophia gets a call from Diane who is from a Missing Person Company (MPC), and she offers to help Sophia. From then on, Sophia visits the MPC and finds a friend in Diane because she is the only one trying to help her, while Paolo insists that the impostor is Sab. Eventually, Sophia finds herself in Diane\u27s office, which how looks like a doctors clinic, and it is revealed to her that after the car accident, she acquired a mental illness called Capgras Syndrome which causes her to believe that her daughter is an impostor. It is revealed in the film that Sophia recovers and recognizes Sab... but not for long. After a few months, she goes through a relapse and cannot recognize her daughter one again

Automated soap-less dirt and stain removing ultrasonic dishwasher

Dishwasher are starting to become a common sight in everyday normal households, due to this fact there will also be a rise in the demand for soap which will be used in the dishwasher. An increase in the demand for soap will therefore increase the volume of wastewater produced in each of the current households. The researchers addressed this issue by creating a soap-less ultrasonic dishwasher, from the name itself this dishwasher does not use any kind of soap but rather it uses vibrations created from the transducer after were struck with a certain frequency form the signal generator. The system that was developed used piezoelectric transducers which were set at 28 KHz to maximize the cleaning range of the transducer. They were first connected into the ultrasonic generator which produced waves at 28 KHz to turn on the transducers, the transducers then produce the vibrations which therefore start the ultrasonic cavitation process. The ultrasonic cavitation process is the one mainly responsible for the cleaning process, the microscope bubbles strike the utensils thus removing the stains that are present in the utensils. The system also have a solenoid valve and a ball valve connected to it, to aid in the draining and filling up of water. A higher water temperature also aids in the cleaning process

DVD-ROM peripheral for Android OS powered tablets

Being in the information age, mobile devices such as tablets, computers and many electronic devices plays a vital role in our daily lives. We commonly used it as a necessity in the different activities we do. In this research paper we can see that there is a lot more to it than meets the eye, and rather than manipulating the default and pre-defined features of a tablet we ourselves can set in what it does. The primary objective of this study is to have an interconnection between a DVD-ROM drive and a modern tablet. Using a CD/DVD as the instrument of storage we are able to transfer the files from the storage device to the tablet that we have. It would be as surpass that as those of the commercial products and enable a feature that is not currently available but useful especially in emergency situations. It can also be provided as a research data in manipulating the kernel level of android operating system. The materials used are as follows: tablet, DVD-ROM, connectors, and CD/DVD storage devices. From the records gathered and data gathered, the group comes up with a new way of implementing the kernel and stimulating the functions that we wanted it to have. The group concluded that the interconnection is possible as to what the final prototype has shown in the end of this scholarly paper. Different data involving the transfer and the file formal compatibility is shown as vital evidence in proving the solution. For computers being the only device to do it so far shows a barrier being breached making it a great alternative electronic device for people to use which is cheaper and easier to carry. People know that an innovation takes place in the smallest to the largest idea, and to find a good one, we need to think outside the box and create a new concepts to implement and manipulate

Influenza-associated excess mortality in the Philippines, 2006-2015.

Influenza-associated mortality has not been quantified in the Philippines. Here, we constructed multiple negative binomial regression models to estimate the overall and age-specific excess mortality rates (EMRs) associated with influenza in the Philippines from 2006 to 2015. The regression analyses used all-cause mortality as the dependent variable and meteorological controls, time, influenza A and B positivity rates (lagged for up to two time periods), and annual and semiannual cyclical seasonality controls as independent variables. The regression models closely matched observed all-cause mortality. Influenza was estimated to account for a mean of 5,347 excess deaths per year (1.1% of annual all-cause deaths) in the Philippines, most of which (67.1%) occurred in adults aged ≥60 years. Influenza A accounted for 85.7% of all estimated excess influenza deaths. The annual estimated influenza-attributable EMR was 5.09 (95% CI: 2.20-5.09) per 100,000 individuals. The EMR was highest for individuals aged ≥60 years (44.63 [95% CI: 4.51-44.69] per 100,000), second highest for children aged less than 5 years (2.14 [95% CI: 0.44-2.19] per 100,000), and lowest for individuals aged 10 to 19 years (0.48 [95% CI: 0.10-0.50] per 100,000). Estimated numbers of excess influenza-associated deaths were considerably higher than the numbers of influenza deaths registered nationally. Our results suggest that influenza causes considerable mortality in the Philippines-to an extent far greater than observed from national statistics-especially among older adults and young children

A comparative study on the anti-bacterial efficacy of selected acne-preventing sheet masks against Staphylococcus aureus

The antibacterial properties of the different components of anti-acne sheet masks will be determined through the diameter of the inhibitory zones of all serums of the different sheet masks against S. aureus by calculating the diameter of the zone. The diameter can be calculated by drawing contours and setting the appropriate threshold value. It can also determine if the different serums of the sheet masks can eliminate S. aureus using the calculated zone of inhibition and the appropriate standard values. The calculated zone of inhibition will be compared and analyzed using t-test. The different serums will be compared using the analysis of variance (ANOVA). Certain values are gathered from the standard given ranges of antimicrobial agents and their specific zone diameter effectiveness

Improving Practice for Urinary Continence Care on Adult Acute Medical and Rehabilitation Wards: A Multi-Site, Co-Created Implementation Study

Many adult inpatients experience urinary continence issues; however, we lack evidence on effective interventions for inpatient continence care. We conducted a before and after implementation study. We implemented our guideline-based intervention using strategies targeting identified barriers and evaluated the impact on urinary continence care provided by inpatient clinicians. Fifteen wards (acute = 3, rehabilitation = 7, acute and rehabilitation = 5) at 12 hospitals (metropolitan = 4, regional = 8) participated. We screened 2298 consecutive adult medical records for evidence of urinary continence symptoms over three 3-month periods: before implementation (T0: n = 849), after the 6-month implementation period (T1: n = 740), and after a 6-month maintenance period (T2: n = 709). The records of symptomatic inpatients were audited for continence assessment, diagnosis, and management plans. All wards contributed data at T0, and 11/15 wards contributed at T1 and T2 (dropouts due to COVID-19). Approximately 26% of stroke, 33% acute medical, and 50% of rehabilitation inpatients were symptomatic. The proportions of symptomatic patients (T0: n = 283, T1: n = 241, T2: n = 256) receiving recommended care were: assessment T0 = 38%, T1 = 63%, T2 = 68%; diagnosis T0 = 30%, T1 = 70%, T2 = 71%; management plan T0 = 7%, T1 = 24%, T2 = 24%. Overall, there were 4-fold increased odds for receiving assessments and management plans and 6-fold greater odds for diagnosis. These improvements were sustained at T2. This intervention has improved inpatient continence care

Antibiotic Isoflavonoids, Anthraquinones, and Pterocarpanoids from Pigeon Pea (Cajanus cajan L.) Seeds against Multidrug-Resistant Staphylococcus aureus

Cajanus cajan L. (pigeon pea, locally known in the Philippines as kadios) seed is a functional food with health benefits that extend beyond their nutritional value. C. cajan seeds contain highly diverse secondary metabolites with enriched beneficial properties, such as antibacterial, anticancer, and antioxidant activities. However, the antibacterial activities of secondary metabolites from Philippine-grown C. cajan, against multidrug-resistant Staphylococcus aureus have not been thoroughly described. Here, we investigated the in vitro antibacterial properties of C. cajan seed against multidrug-resistant S. aureus ATCC BAA-44 (MDRSA) and three other S. aureus strains (S. aureus ATCC 25923, S. aureus ATCC 6538, and coagulase-negative S. aureus) and, subsequently, identified the antibiotic markers against S. aureus strains using mass spectrometry. Secondary metabolites from C. cajan seeds were extracted using acetone, methanol, or 95% ethanol. Antibacterial screening revealed antibiotic activity for the C. cajan acetone extract. Bioassay-guided purification of the C. cajan acetone extract afforded three semi-pure high-performance liquid chromatography (HPLC) fractions exhibiting 32–64 µg/mL minimum inhibitory concentration (MIC) against MDRSA. Chemical profiling of these fractions using liquid chromatography mass spectrometry (LCMS) identified six compounds that are antibacterial against MDRSA. High-resolution mass spectrometry (HRMS), MS/MS, and dereplication using Global Natural Products Social Molecular Networking (GNPS)™, and National Institute of Standards and Technology (NIST) Library identified the metabolites as rhein, formononetin, laccaic acid D, crotafuran E, ayamenin A, and biochanin A. These isoflavonoids, anthraquinones, and pterocarpanoids from C. cajan seeds are potential bioactive compounds against S. aureus, including the multidrug-resistant strains

Corrigendum to: Rice Galaxy: an open resource for plant science

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